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The first step would be to
remove your standard brightfield objectives from the microscope
and return them to their plastic containers.
Next, install your Phase
Contrast objectives in the nosepiece in their proper order:
10X, 20X, 40X & 100X, for example.
Keep the plastic containers
on hand for your phase objectives.
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The standard Abbe Condenser
needs to be removed. Condensers cannot be removed unless
the stage is racked up all the way and the condenser rack
is lowered all the way.
Next, unscrew
the chrome locking thumbscrew which is on the right-hand
side behind the chrome centering screw as shown in the picture
at left. |
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The condenser
should be loose at this point and simply pull the unit
straight out the front.
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The Zernike
Condenser can now go in place. |
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Tighten the locking
thumbscrew snugly and then rack the condenser up all the
way.
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Next, we will center the
iris. Turn on the power switch and adjust the brightness
knob to a comfortable level.
Rotate the Zernike condenser
settings wheel so that the "A" position is engaged
as shown in the picture at left.
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Rotate the 10X objective
into the working position. Close the "Iris Adjustment"
by cranking the knob all the way to the left. When looking
into the eyepieces, one will see the aperture hole of
the diaphragm. It may or may not be centered in the field
of view or FOV. You may also need to focus the microscope
so the edges of the aperture hole are clear to see.
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Place each hand on each of the
"Condenser Centering Screws" while at the same
time, looking into the eyepieces and adjusting the centering
screws until the aperture hole is in the center of the FOV. |
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When centered, the aperture
will be in the center of the field of view as demonstrated
in the picture at left. |
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With
the 10X objective still in the working position, rotate
the Zernike Condenser control until the "Ph1"
position is engaged as shown. |
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Next,
check to see if the set screws on the bottom of the Zernike
Condenser are loosened.
The
set screws will need to be loosened for the larger adjustment
knobs to be able to adjust. |
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Next,
remove the right eyepiece and in its place, insert the centering
telescope.
The
top of the centering telescope has a focus adjustment which
will be held in one hand with the other hand holding the
body.
While
looking into the centering telescope, adjust the focus so
you may get a sharp image of the phase annuli to be adjusted. |
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When
the annuli are not aligned, one will see an image similar
to the picture at left: one light ring is not aligned with
the other. |
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To
bring the annuli into alignment, place each hand on each
annulus adjustment knob and while looking into the eyetubes,
bring the annuli into common alignment. |
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When
the annuli are aligned, one will see an image similar to
the photo at left.
Each
light ring is imposed on the other making one aligned ring. |
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The
"Ph1" position is the annulus for the 10X objective.
The other positions are:
Ph2
= 20X and 40X Phase Annulus
Ph3 = 100X Phase Annulus
DF = Darkfield Stop for 40X Darkfield Objective
C = Closed (For MX6000 Epi-Fluorescence)
A = Aperture Iris ( For Brightfield work) |
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Repeat
the annulus alignment for the 20X/40X and the 100X objectives
by using the Ph2 and Ph3 settings on the Zernike Condenser.
When
finished, lock down the settings by tightening the "set
screws" as shown in the picture at left.
The
annulus adjustments should be checked occasionally or when
accessories are swapped. |
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