Fluorescence microscopes - fine art restoration / conservation




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Fine Art Restoration / Conservation


Fine Art Restoration / Conservation

Fluorescence microscopes

The microscope shown below has a digital camera fitted to a vertical phototube:

This microscope has been specially designed to provide, at an economical price, instrument for examining paint samples.

It is equipped with 10x, 20x and 40x objectives and epi illumination. These objectives are for brightfield and darkfield. In brightfield mode the illumination is directly through the objective. In darkfield mode, the illumination comes down, round the objective and falls on the specimen obliquely. The central imaging part of the brightfield/darkfield objective picks up light scattered by the specimen. This illumination technique can be particularly beneficial when viewing low reflectivity specimens. The illumination itself is provided by a halogen lamp, positioned to the rear of the microscope.

The microscope can also be used for epi-fluorescence work, when intense lower wavelength light is used to illuminate the specimen. Certain chemicals have characteristic longer wavelength fluorescence. A built-in barrier filter (sometimes called emitter filter) cuts out the excitation light and enables the fluorescence to be observed. The microscope can be switched to this mode operation by operating a slider, which directs (via a mirror) the Mercury arc fluorescence illumination into the epi condenser. The mercury arc lamp unit can be seen to the side of the microscope.

Using 10x eyepieces, the magnifications of 100x, 200x and 400x are obtained.


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