Particular
compound microscope objectives have a focusing ring that
can be found when unscrewing the objective cover. Parfocalling
microscope objectives is a procedure by which each objective
is adjusted to remain in relative focus with the other.
If each objective is not parfocalled, an object would not
remain in clear focus when switching powers.
Not
only is this inconvenient, it can increase eye strain and
some wear and tear on your microscope. You may find parfocalling
objectives too hard for you to perform yourself. Fortunately,
most Meiji dealers have service persons on staff who can
perform this task for you. But if you are mechanically inclined,
the following procedure can be used as a general guide.
Consequently, Meiji Techno assumes no liability in regards
to the outcome of end users who disassemble objectives to
perform this or other tasks. So be careful!
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Remove the objectives to
be parfocalled.
Each objective to be parfocalled
will be removed and the outer covers opened.
If you have a 4X or 10X objective
in your nosepiece, leave them screwed in. They do not have
user adjustable settings.
The higher power objectives
will be parfocalled starting with the objectives higher
than 10X. The 10X will essentially be the starting point
to which the other objectives will be adjusted.
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Remove
the outer objective cover.
The covers unscrews counter-clockwise
like most threaded devices and should unthread pretty easily.
Keep track which covers go
with which objectives !
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This
ring is the objective parfocal adjustment.
See if you can turn the ring.
The ring may or may not have
a dab or drop of optical cement holding it in place.
You may have to look very carefully
to find the cemented spot.
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Remove
the optical cement securing the adjustment ring.
Using acetone and a lightly
dampened Q-tip, carefully remove the dab of cement holding
the ring in place. You may have to work the ring back and
forth to make the ring spin freely and to remove the debris
in the threads.
After each ring is free, re-install
the objectives ( without the covers on) on the microscope
in their proper order of magnitude.
Rotate the 10X objective into
working position and focus on your standard. We used a stage
micrometer but you can use anything flat that has fine lines
on it. Now switch to the next objective.
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While looking through the microscope,
rotate the adjustment ring of the objective.
Starting with the next objective,
probably the 40X, adjust the ring while looking through
the scope.
BE CAREFUL !
The adjustment is rather touchy. You may miss it if you
blink your eye or if you are turning the adjustment too
quickly.
Repeat the process for the
rest of your objectives. When you are done, you should be
able to quickly cycle through all of the objectives without
having to re-focus.
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Apply optical cement to
secure the objective adjustment.
When your objectives are all
adjusted, you may want to secure them so they do not fall
out of adjustment due to vibration or regular usage.
If you do not have optical
cement, a small dot of clear nail polish can do the trick.
You can add the cement while
the objectives are still screwed into the nosepiece. This
photo shows where the cement is applied. Allow the cement
to dry and re-install the objective covers.
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The
microscope objectives are now "parfocalled" and
they should hold their focus each time you change powers.
The exception would be the 4X objective. The 4X objective
is your "scanner"; it's used to locate the general
area of interest and not much else. The 40X and 100X should
be parfocal with the 10X objective. Since the 10X objective
does not have an adjustment ring, all other objectives that
are adjustable use the 10X as a starting point. Your particular
microscope may have a different number of objectives with
different powers. However, the procedure will still remain
the same.
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