Parfocalling is a procedure by which
a microscope is adjusted to remain in focus throughout the magnification
range. If the microscope is not parfocalled, an object in clear
focus at one magnification will quickly go out of focus as you zoom
to a higher or lower magnification, requiring you to zoom and focus
at the same time. Not only is this inconvenient, it also increases
strain on your eyes and wear and tear on the microscope. Fortunately,
parfocal adjustment on a zoom stereo microscope is quick and easy!
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Set the correct interpupillary
distance by moving the eyetubes closer together or farther
apart to match your individual setting.
You can tell when the setting is right when you are able to
look into the eyetubes and see the image framed on ONE circle.
When done properly, your eyes
will quickly and easily register the circle. Your eyes are
very adaptable so if the interpupillary distance is off a
little bit, your eyes will adjust to it. However, this increases
strain on your eyes. To avoid eye strain, look up from the
microscope at a distant object for a moment and then look
back into the eyepieces. The circle should appear quickly
and easily. If not, re-adjust the interpupillary distance
until it does.
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Set
the dioptric adjustment rings on both eyepieces to zero.
Line up the marks as shown in
the photo.
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Place
a specimen you wish to examine under the microscope.
We are going to choose a coin
but you can use any flat specimen that does not have a lot
of depth.
Samples with easily identifiable
features will allow us to quickly determine focusing. |
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Zoom the microscope
to the highest setting (4.5X) and bring the image in the
eyepieces into clear focus using the large black focus knob
on your stand or focus block.
(Not the
zoom knob with the numbers on it.)
Depending on your model, you
may have two focus knobs, one for course adjustment and one
for fine adjustment. |
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Zoom the microscope
to the lowest magnification (0.7X).
The
image may now be slightly out-of-focus but DO NOT re-adjust
the large focus knob. |
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Instead, while looking into
the microscope, close your left eye and while adjusting
the right eyepiece, bring the specimen into focus with your
right eye ONLY while adjusting the diopter as shown in the
photo.
When the image of your specimen
is clearly in focus in the right eyepiece, close your right
eye and repeat the procedure until the left eyetube is
focused clearly.
Just as we did for adjusting
the interpupillary distance, use the same "look-away"
technique to ensure that focusing through each tube is quick
and easy.
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The microscope is now "parfocalled"
and should hold focus throughout the entire zoom range. You may notice
that a microscope parfocalled by someone else is NOT exactly parfocalled
for you. This is because each person has different focusing abilities:
i.e. different interpupillary distance, some people wear glasses,
etc. In this sense, parfocalling means adjusting the microscope to
fit you and it is well worth the effort.
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